Re: FITC-labelling of bacteria
From: Adrian Robins (adrian.robins@nottingham.ac.uk)
Date: Wed Jun 26 2002 - 03:33:22 EST
David
we have used CFSE with bacteria (see Logan RPH, Robins A, Turner GA,
Cockayne A, Borriello SP, Hawkey CJ. A novel flow cytometric assay
for quantitating adherence of Helicobacter pylori to gastric epithelial
cells. Journal of Immunological Methods 1998; 213:
19-30 for our experience with Helicobacter). I think longevity of
staining will depend on how fast your bugs divide.
best regards
Adrian Robins
At 11:42 25/06/02 -0500, you wrote:
Mike:
> I have used Molecular Probes Cell Tracker green on viable bacteria
for
> cell binding experiments using flow for quant. This worked
very well
> for me.
In your hands, how long did the dye remain detectable? I'm
thinking of a
phagocytosis experiement involving Staph aureus and macrophages, so I
need
the bugs to be "detectable" for a long time (>6 hour if not
days). I need
to do some leg work but do you or anyone know if CSFE can be used
in
bacteria?
Many thanks
David
Dr R. Adrian Robins
Reader in Immunology
Division of Immunology
'A' floor West Block
Queens Medical Centre
Nottingham NG7 2UH
Phone 0115 970 9123
Fax 0115 970 9125
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