I have used Molecular Probes Cell Tracker green on viable bacteria for
cell binding experiments using flow for quant. This worked very well
for me.
Hazel Davey wrote:
>
> On 12 Jun 2002 at 9:15, Josef Azem wrote:
> >
> > Dear Flowers!
> >
> > Has anyone have a protocoll for FITC-labelling of bacteria?
>
> Straight FITC rather than FITC labelled antibody?
>
> Here is the method I use:
>
> Stock solution of FITC is 1 mg/ml FITC in acetone. Final
> concentration of FITC incubated with bacteria should be in the 1-10
> ug range depending on sample / cytometer. Incubation for 15 mins is
> usually sufficient. I think I adapted this from Miller, J.S. and
> Quarles, J.M., 1990. Flow cytometric identification of microorganisms
> by dual staining with FITC and PI. Cytometry 11, 667-675.
>
> As usual you may have to adjust this for your circumstances but this
> should provide a useful stsrting point.
>
> Hazel--
> Hazel Davey HLR@aber.ac.uk http://pcfcij.dbs.aber.ac.uk/
> Sefydliad y Gwyddorau Biolegol, Prifysgol Cymru, Aberystwyth
> Inst. Biological Sciences, University of Wales, Aberystwyth
> http://www.nspcc.org.uk/donate-4-free/donate-mainhome.asp
--
________________________________________________________
/ Michael J. Herron, U of MN, Dept. of Pediatrics/BMT /
/ herro001@umn.edu /
/ 612-626-4321 Mpls MN 55455 /
/_______________________________________________________/
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