On 12 Jun 2002 at 9:15, Josef Azem wrote: > > Dear Flowers! > > Has anyone have a protocoll for FITC-labelling of bacteria? Straight FITC rather than FITC labelled antibody? Here is the method I use: Stock solution of FITC is 1 mg/ml FITC in acetone. Final concentration of FITC incubated with bacteria should be in the 1-10 ug range depending on sample / cytometer. Incubation for 15 mins is usually sufficient. I think I adapted this from Miller, J.S. and Quarles, J.M., 1990. Flow cytometric identification of microorganisms by dual staining with FITC and PI. Cytometry 11, 667-675. As usual you may have to adjust this for your circumstances but this should provide a useful stsrting point. Hazel-- Hazel Davey HLR@aber.ac.uk http://pcfcij.dbs.aber.ac.uk/ Sefydliad y Gwyddorau Biolegol, Prifysgol Cymru, Aberystwyth Inst. Biological Sciences, University of Wales, Aberystwyth http://www.nspcc.org.uk/donate-4-free/donate-mainhome.asp
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