Hi Marty, Just to reassure you, FCS Express does use the linear model correctly. It may shock you to learn that other people, aside from your friends at Treestar, can read Dr. Bagwell's paper and actually understand it enough to implement the algorithms that he describes. FCS Express allows you to enter the files with the single stained controls and it calculates the matrix values based upon the data in the file. In addition, in order to see how slight changes in the compensation can distort your data, you can change the compensation values with sliders to see what happens. Its a great for teaching people the difference between proper, over- and under- compensated data. You can also enter a user defined value to smooth out the digitization errors that can occur in the low end of lower resolution data. If anyone finds any problems with the compensation (which hasn't happened yet), please let me know and of course I will fix any errors. But until that happens, I think that your assumption that FCS Express uses the linear model correctly is actually a very good one. -Dave At 06:23 PM 6/12/02 -0700, you wrote: >Lastly, of course, this assumes that Summit (and FCS-Express) do use >the linear model correctly. In an older version of Summit I used, >overcompensation resulted in data "bouncing" off the axis instead of >being smashed down on the axis.
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