mRNA loss during cell fixation

From: Richard Haugland (richard.haugland@probes.com)
Date: Sat Dec 28 2002 - 12:14:10 EST


Does anyone know how much mRNA (what percentage) is lost to the medium
during the typical process of cell fixation and permeabilization when
one tries to do in situ mRNA detection? Are there any authoritative
studies and references on this?

Thanks



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