Hi Paul, although we have not sorted CHO cells post detachment nor tested VE-cadherin, we have recently had success detaching the CHO cells from culture dishes using a fairly new product called Accutase from Pheonix Flow Systems. The active enzyme(s) in this product are not disclosed (see their web-site for pdf of this product) but in a preliminary experiment we obtained a single cell suspension that exhibited very high viability at all concentrations tested. In one parallel experiment using the primitive hematopoietic cell line KG1a, we noted that surface expression of CD34 (class III epitope), CD109 and CD45 (J33, pan CD45) were not cleaved at concentrations sufficient to effect detachment of CHO cells. Might be worth a try. Rob Sutherland University Health Network Toronto
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:31 EST