Thank you for all your answers, concerning detection of little T-cell specific response. <10 000. Briefly, besides ELISPOT there are at least two methods challenging this problem. 1. A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens. J Immunol Methods. 1999 Nov 19;230(1-2):99-112. 2. Multiparameter cytokine-specific affinity matrix assay for the determination of frequencies and phenotype of antigen-reactive T cells.J Immunol Methods. 2002 Feb 1;260(1- 2):37-42. Stoyan Klinische Forschergruppe Neuroendokrinologie Medizinische Universität Lübeck Ratzeburger Allee 160 Haus 23a 23538 Lübeck Germany Tel.: +49 (451) 500-3644 Fax: +49 (451) 500-3640
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