> -----Original Message----- > From: Rene Rückert [mailto:rrueckert@fz-borstel.de] > Sent: Tuesday, April 23, 2002 3:16 AM > To: Cytometry Mailing List > Subject: Surface + Apoptosis detection > > I'd like to detect apoptosis of murine dendritic cells and > T-cells using > anexinV / PI staining (using a BenderMedsystem kit). Cells are > co-cultured for 24h. For cell discrimination, the surface should be > stained with CD11c or CD8/CD4 APC (for detection on standard two-laser > Calibur). Anybody did this before? Should I stain first with the > APC-labelled antibodies? Or first Annexin? And wash > thereafter? Or both > together? And PI before analysis? > Any suggestions? Hints? For a study in which we combined TUNEL with surface phebnotyping, you can see our paper: Blood 92:4230-4237, 1998 and for annexin with surface labeling , please see our paper: Clin Diag Lab Immunol, 8:74-78, 2001 Good luck, Tom **************************************************************************** * Thomas W. Mc Closkey, Ph. D. Director of Flow Cytometry, North Shore University Hospital Assistant Professor of Pediatrics, New York University School of Medicine Boas Marks Biomedical Research Center, 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866 **************************************************************************** *
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