Our studies in the last year have convinced me that CD163 is the best single marker for human monocytes, followed by CD64, then CD14. The combination of CD163PE and CD14FITC or CD14PE maybe superior to single reagents if your desire is to identify the most monocytes. The first two markers can be obtained as PE and FITC conjugates from Maine Biotechnology Services (www.mainebiotechnology.com) and CD14 can be obtained from any of the usual suspects. Good luck, Bruce H. Davis, M.D. Trillium Diagnostics, LLC c/o Maine Medical Center Research Institute 81 Research Drive Scarborough, Maine 04074 USA PHONE: 207-885-8113 FAX: 207-885-8110 Email: davisb@mmc.org >>> "Oughton, Julie" <julie.oughton@orst.edu> 08/29/02 04:28PM >>> Hi! I am forwarding a request from an investigator who uses our 4-color Coulter XL flow cytometer. Our core has lots of experience in phenotyping mouse blood, but little to no experience with human blood. And we are finding it more difficult to identify monocytes in human blood than in mouse blood. Therefore, we are seeking advice/suggestions. Her request is below. You may reply to me and I will forward all messages to her. Thanks in advance Julie Oughton Oregon State University ********************************************************************* We are trying to determine leukocyte counts and differentials in FROZEN human whole blood samples and are looking for some feedback/input. We are currently using CD45, CD15 and CD11b in order to enumerate monocytes and granulocytes, but we are having some trouble. It appears that CD11b is also staining some of our lymphocytes. We are considering adding CD14 to look at monocytes, but are wondering if this is the best marker to identify monocytes. We are also considering dropping CD11b or replacing it with another marker. All suggestions would be greatly appreciated. Angela Mastaloudis PhD Graduate Student Linus Pauling Institute Oregon State University 737-9376 Lab) 737-6790 (office)
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