Hi all, I'm trying to detect an intracellular antigen on a monocytic cell line using an unlabelled primary followed by a RPE labelled seconday so am anticipating a lot of background. I know I have to block Fc receptors to reduce background but can I do this after my fixation/permeabilisation step? Also, I come from a immunohistochemistry background it makes sense to me to also use a blocking step which caters for non-specific binding of the antibody - is it possible to do this (perhaps in conjunction with the Fc blocking step)? I'd appreciate any tips. Thanks, Elaine Kennedy -------------------------------------------------------------------- This message, including attached files, may contain confidential information and is intended only for the use by the individual and/or the entity to which it is addressed. Any unauthorized use, dissemination of, or copying of the information contained herein is not allowed and may lead to irreparable harm and damage for which you may be held liable. If you receive this message in error or if it is intended for someone else please notify the sender by returning this e-mail immediately and delete the message. --------------------------------------------------------------------
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