We have today completely updated my Handbook at our Web site. This will be essentially equivalent to what the 1000+-page print version of the Handbook will be when it is available in early fall. We hope to also have this available on CD in searchable pdf files sooner. http://www.probes.com/handbook/ We have also today released a particularly important and versatile new technology for antibody labeling for imaging and flow cytometry that we call Zenon Technology. This permits quantitative preparation of fluorescent complexes of mouse IgG1 antibodies (and at least some IgG2 mouse antibodies with some crossreactivity with human antibodies) in <10 minutes using even submicrograms of the monoclonal and use of multiple unlabeled mouse monoclonals in the same experiment. Some particularly unique features of Zenon labeling are the ability to tune the fluorescence intensity to some extent, lack of interference from albumins in the solution and the ability to label many antibodies that are not available as direct conjugates. Brightness of the Zenon complexes is about the same as that of direct antibody conjugates. One can change colors and color combinations at will and pretty much optimize the labeling in minutes. We are now developing Zenon labeling reagents for other isotypes and species. So far the Zenon method works best for imaging and flow but we have also used it for some tissue labeling, including using mouse monoclonals on mouse tissue. This is described in general at http://www.probes.com/products/zenon/ and in greater detail at http://www.probes.com/handbook/sections/0702.html
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