This is a question for the Clinical people involved in breast cancer prognostic indicators: What methods are your labs using for an indication of proliferate rate for breast cancer. We have done (and are still currently doing) DNA ploidy and S-phase analysis. This is a logistical headache, and no longer the method of choice for many labs. So - for those of you no longer doing DNA analysis....what are you doing? Immunohistochemistry? MIB-1? Ki-67 by flow? ...by immuno ? If by immunohistochemistry, who does the counting? Do you give the clinician a hard number? What kind of numbers do you turn out? What kind of study (cut offs) did you do to come up with ranges? Thank you. Kathy Altig Southwest Washington Medical Center Vancouver, WA
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