If you sort a certain number say 30 onto a slide then count under the microscope how many do you get? If you dont get very close to 30 then your drop delay is out of whack. If you get close to 30 every time then some of you beads are probably sticking to the tube, tubing etc. I wouldn't expect to see all the beads, 15000/30000 sounds about right unless you are very careful, washing out the tube and tubing with lots of clean sheath etc Best. Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> "McDonald, Ken" <ken_mcdonald@Merck.com> - 11/27/02 5:41 PM >>> Hi All, I'm seeing a discrepancy in what the Vantage sorter tells me it's sorted and the actual numbers sorted. For example, I sorted for green calibite beads (30,000) and when I re-ran them to check for purity there were roughly 15,000 only. Does anyone have the same problem? The purity is good (99%) and everything else checks out by the book?..Thanks in advance..Ken Ken McDonald (Biologist) Merck Frosst Canada Tel: 514-428-2802 email: ken_mcdonald@merck.com www.merckfrosstlab.ca ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (Whitehouse Station, New Jersey, USA) that may be confidential, proprietary copyrighted and/or legally privileged, and is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please immediately return this by e-mail and then delete it. ==============================================================================
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