Dear Marj, We perform monitoring of these transplant patients in our lab too. Initial samples are setup using a routinely prepared cocktail of CD3/CD4/CD8/CD45 and CD3/NK/19/45, fluorescence gated, and analyzed for Total T (CD3), T-Helper (3+4+),T-Cytotoxic/Suppressor (3+8+), true NK (3-NK+), and B-cells (3-19+). Followup (post Immune-suppression therapy) samples are set up with CD3/CD2/CD45, fluorescence gated, and analyzed for true T (CD3+CD2+) as well as T-NK (CD2+CD3-). The baseline results versus the follow-up studies can then be compared for thymoglobulin efficacy since large increases in CD2+3- (normally NK cells) may indicate the presence of T cells that have lost their CD3 recognition but are still in circulation. This is simple and easy to use as a monitoring panel of Transplant therapy. Sincerely, Bruce Bruce Greig Flow Cytometry Services Vanderbilt University Medical Center Nashville, TN. 37232 (615)322-2682
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