Hi Robert, I would suggest Annexin-V / PI as a first flow cytometry assay for detection of apoptosis, especially, when suspension cell cultures are used: Just pipette cells from the cutures into FACS tubes, add annexin-V-FITC and PI and analyse. We have done this frequently and with good results. We did not use special Annexin staining buffers coming with the kits because Ca++ is already in the medium. We also omitted washing steps from the standard Annexin staining protocols. Best regards Georg
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