Important tip! Colleagues of ours have just warned us that Miltenyi switched the clone of anti-human CD8 that they use with their anti-CD8 beads. The previous one (BD's SK1) works well with rhesus macacques, but the current one (BW135/80) apparently does not. Our colleagues unfortunately spent several months trying to diagnose the problem before they found out (with some probing) that Miltenyi changed antibodies almost 6 months ago without checking if the clone cross-reacts to rhesus. Based on the laboratory experience, the guess is that it very poorly cross reacts (if at all). mr
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