a question on cell cycle analysis with PI

From: Yalin Guo (guo@mm11.ukl.uni-freiburg.de)
Date: Tue Mar 26 2002 - 09:12:44 EST 

I am doing a cell cycle analysis using cultured and non-cultured
human CD34+ cells. I got a protocol with PI staining (0.1% Na
Citrate, 0.1% Triton X-100, 20 ug/ml PI), but without fixation
and RNase. I should be very grateful to get any suggestions for
this method.

Yalin

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