Well 6 times is a bit exagerated, but we recently labelled some anti-mouse-CD-25 with Alexa 488 and gained one log compared to the commercial FITC and PE products.... So I think it's worth labelling with Alexa instead of FITC, it's as easy, a bit more expensive, but for certain antigens worth the trial ! Best, Matthias > > On Molecular Probes' website are claims that Alexa 488 is 6-7 times brighter > than FITC. Preliminary evidence from our conjugations of the same antibody > with FITC or Alexa 488 indicate no advantage from using Alexa 488. Can > anyone who compared Alexa 488 and FITC share their findings? >
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