Dear ISAC Congress Participants, all investigators interested in the assessment of antigen specific lymphocytes (and Tony Bakke see below). Please bear witness to the Workshop A-V chaired by Maurice (Mo) O'Gorman:"Analysis of Immune Specific Responses" to be presented Sunday evening, May 5th from 5 until 7pm (immediately before the opening reception). Room assignment not yet available. The workshop will feature formal presentations and discussions by and with 4 well known experts directly involved with applications using tetramers, dimers and tracking dyes to investigate antigen specific lymphocytes. Dr. John Altman. Updates in the design application of tetramer staining Dr. Albert Donnenberg. Assessing Sensitivity and Specificity of MHC-Tetramer Data Dr. Jonathan Schneck. Recent experience in the development and use of dimeric antigen-specific T cell reagents Dr. Andrew Wells: Monitoring specific lymphocyte immune responses in vitro and in vivo using fluorescent tracking dyes. Each speaker will present a 15 -20 minute lecture and will be available to answer questions, including the question below, posed by Dr. Bakke. If you have issues and concerns with applications involving these technologies, this is your chance to discuss these issues with individuals directly involved in their development and application Regards Maurice (Mo) O'Gorman. From Dr. Bakke: <<Dear all, Our tetramer staining is giving significant background staining in people who are negative for the HLA-A2 allele and negative for exposure. The background is low intensity. Any suggestions about blocking this background staining? Thank you, Tony Bakke>> -- ******************************************************************************###################################################################******************************************************************************- -- Maurice (Mo) R.G. O'Gorman, Ph.D., D(ABMLI) Associate Professor Pediatrics Northwestern University Medical School Director Diagnostic Immunology Laboratory Children's Memorial Hospital 2300 Children's Plaza, MB#50 Chicago, IL 60614 Ph 773 880 3070 fax 773 880 3739 lab ph 773 880 4361 Editor in Chief - Clinical and Applied Immunology Reviews (CAIR) Elsevier Sciences www.elsevier.com/locate/cair
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