Hi all, we mentioned some differences using isotype controls over the time. A year ago, we started to analyse intracellular interleukins in monocytes after LPS stimulattion. At the beginning, we always had isotyp controls for stimulated and non-stimulated cells. But we did not see a difference between these controls (using PE-labeled antibodies from Pharmingen). After some time, the user decided to use only one control. We now repeated both controls and realised indeed a difference between non-stimulated cells and stimulated cells (3 hours LPS, getting higher mean values in comparison to non-stimulated isotype controls). What's the overall experience? Do everybody use both isotype controls and did you see allways different mean values for these controls? Thanks for your help Andreas ___________________________________________________ Andreas Simm Klinik fuer Herz- und Thoraxchirurgie Universitaet Halle Ernst-Grube-Str. 40 06120 Halle Tel.: +49 (0) 345 557 2647 FAX: +49 (0) 345 557 2782 e-mail: andreas.simm@medizin.uni-halle.de
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