Hello Flow-ers, I was hoping someone out there could lead me in the right direction. I was interested in finding an injectable fluorescent dye (into mice) that would hopefully label cells in a concentration dependent manner so that cells local to the site of injection would be labelled more intensely than more distant cells. My goal is to find a way to label resident cells and track their movement. It doesn't have to be a long term label. I'm trying to look at peritoneal exudate cells and what happens to them upon bacterial infection in vivo. I'm completely lost on a dye that would label preferably in something other than FL-1 and yet would not be too toxic to be used in such a way. Maybe someone out there has a protocol for something like this. I would deeply appreciate any help in this endeavour. thanks -- Wayne Harris Lead Research Specialist Department of Microbiology and Immunology Emory University , School of Medicine
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