Detection of GFP in tissue sections

From: Richard K. Meister (meister.1@osu.edu)
Date: Thu Apr 18 2002 - 13:12:53 EST


Hello, everyone:

I have a client who needs to detect GFP in fixed tissue sections (various
animal tissues) and he is concerned that the fixation process might effect
the fluorescence of the GFP.  I haven't done GFP in tissue, but I have
routinely with fixed flow specimens - and they work just fine.
Nevertheless, he is concerned that he might miss some GFP-labelled (but
non-fluorescent) sites.  So, he is thinking of coming in with a goat
anti-GFP antiserum, followed by an anti-goat 2nd antibody:PE.

So, he has a few questions.  First, is there a particular fixation protocol
that he should followfor GFP-labelled tissue?

Second, what anibodies do you recommend?  And what is your staining
protocol?  Any other tips?

Thanks in advance.

Rick Meister
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*  Richard K. Meister                  Email:  meister.1@osu.edu  *
*  The Ohio State University           Voice:  (614) 292-9716     *
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