Hello, everyone: I have a client who needs to detect GFP in fixed tissue sections (various animal tissues) and he is concerned that the fixation process might effect the fluorescence of the GFP. I haven't done GFP in tissue, but I have routinely with fixed flow specimens - and they work just fine. Nevertheless, he is concerned that he might miss some GFP-labelled (but non-fluorescent) sites. So, he is thinking of coming in with a goat anti-GFP antiserum, followed by an anti-goat 2nd antibody:PE. So, he has a few questions. First, is there a particular fixation protocol that he should followfor GFP-labelled tissue? Second, what anibodies do you recommend? And what is your staining protocol? Any other tips? Thanks in advance. Rick Meister * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Richard K. Meister Email: meister.1@osu.edu * * The Ohio State University Voice: (614) 292-9716 * * Dept. of Veterinary Biosciences FAX: (614) 292-6473 * * Cytometry Instrumentation Lab * * 1925 Coffey Road * * Columbus, OH 43210 U.S.A. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
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