Hi HLA Flow Cytometrists, We have noticed in the CAPS Flow Crossmatch surveys that 75% of the labs are diluting their serum and 25% uses it as neat serum. We were wondering ...1) what serum (or sera) is being used as negative control, 2) what diluent is being used, and 3)whether the negative control serum was also diluted as well. Thanks for your help. Regards, Vivian Wu Immunology Laboratory Vancouver General Hospital, B.C. Canada
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