A couple of weeks back, Dave Coder contributed:
> As a general case, knowing the emission spectrum of the dye that you want to
> detect is a good start. (Emission spectral shifts after conjugating to
> protein occur, but for a first approximation you can ignore the shift if you
> have only the dye spectrum as excited by your laser line.) There are lots
> of sources of this information (Molecular Probes, Chroma Technologies, the
> java app that Joe Trotter did, etc.)
Can anyone tell us about "the java app that Joe Trotter did" ?
Frank Battye.
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