There is a complete protocol in Current Protocols in Cytometry, information for which can be found at http://www.wiley.com/legacy/cp/cpc/ the unit detailing CSFE is 9.11 Flow Cytometric Analysis of Cell Division by Dye Dilution written by Dr. Bruce Lyons Best wishes J. Paul Robinson, Editor Current Protocols in Cytometry Purdue University On 4 Feb 2002, at 9:16, Zenaida Garcia wrote: Does anyone have a working LPA procedure by FLOW using CFSE? I've tried staining human PBMCs and tested the range from 0.25 to 2.5 uM (final concentration). I'm having difficulty compensating as it crosses onto FL2 and FL3. Your help would be greatly appreciated. Zeny J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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