Marty Bigos wrote- >I know it looks fairly bleak from the excitation/emission spectra, >but i wonder if anyone has had success(or failures!) using indo-1 >excited by the 407 nm krypton line. Thanks for any information on >this. I am assured by people who design calcium probes that, not only can't you excite indo-1 with 407 nm, you can't even make a derivative of indo-1 that would excite at 407 nm - or even at 385 nm, which is now the shortest wavelength you can get from a violet (ultraviolet, at this wavelength) diode laser. I have also been told that it might be possible to get an emission ratiometric calcium probe derived from indo-1 that would work with 370 nm excitation from a frequency-doubled, diode-pumped Alexandrite laser. -Howard
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