Beth, My experience has been that a higher power UV laser is beneficial in identifying SP cells by hoechst staining. I worked with a Vantage equipped with an enterprise laser and I found that 40-50 mw power was a bit inadequate for best separation of SP population. I have an Innova 90 Coherent laser ,on a Facs DiVa Vantage SE, running at 150mw which I find much better in producing well defined SP population. Of course, optics and good alignment of instrument will help as well. David Hi, Our lab is going to start using Margaret Goodells method to look at SP cells. For those of you already doing this can you tell me what type of laser you use for UV? I am looking at using either an Enterprise laser with 488nm and UV or having separate UV and 488nm lasers. Thank you, Beth Whalen Manager, Flow Cytometry Core Facility McDonald Research Laboratories/The iCAPTURE Centre St. Paul's Hospital - University of British Columbia 1081 Burrard Street, Vancouver, BC V6Z 1Y6 P: 604-806-8328 F: 604-806-8351 -- David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 Massachusetts General Hospital-East 149 13th Street Charlestown, MA 02129 Tel (617)-726-1683 Fax (617)-724-3164
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