Thanks for all the suggestions and help. Ger > > > > >We are looking for a reliable apoptotic assay for Balb/c 3T3 > >(fibroblasts). We have tried the Annexin V FITC/PI with little success. > >After inducing apoptosis by serum removal we get PI +ve, but FITC -ve. > >We've tried various methods of inducing apoposis, but got little or no > >FITC +ve. We detach the cells using trypsin. > > > >Any suggestions/ help would be appreciated. > > > > > >Thanking you, > >Ger -- ---------------------------------------------------------------------- Gerard Bowe ECVAM Institute for Health and Consumer Protection (IHCP) Joint Research Centre TP. 580 21020 Ispra (VA), Italy Tel: + 39 0332 785122 Fax: + 39 0332 785336 E-mail: gerard.bowe@jrc.it ----------------------------------------------------------------------
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