Pluronic acid will dissolve much easier in DMSO for your assay. Place in 37oC water bath to help dissolve. Need to check your total DMSO content though, cells aren't that tolerant. Hope that's helpful Simon GlaxoSmithKline R&D UK gio@biocfarm.unibo.it 19-Dec-2002 12:07 To: "Cytometry Mailing List" cc: Subject: pluronic acid Someone has suggestion about preparation of 20% pluronic acid for calcium assay? We tried in water but we only arrive to a beker full of foam! Merry christmas and happy new year to everyone, Giovanna Farruggia Dott. Giovanna Farruggia Dip. Biochimica "G. Moruzzi" Sezione di Biochimica Farmaceutica Via S.Donato 19/2 40127 Bologna tel. 051 253562 fax 051 242974
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