Does anyone out there in flowland have experience with the TUNEL assay for apoptosis? I have been using the APO-BRDU kit from Pharmingen, and I have been having trouble with large variations between duplicates. Specifically, I am using duplicate spinner cultures of transfected CHO cell lines. In several experiments, one duplicate had a very low percentage of FITC-positive cells, and the other had a very high percentage. This is true of two different cell lines I have been examining, under both apoptosis-inducing conditions (sodium butyrate exposure) and "regular" (extended batch culture until most of the cells are dead) culture conditions. Any suggestions would be welcome. Shari Brezinsky Biogen, Inc.
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