Re: FITC-labelling of bacteria

From: Keith Bahjat (me@keithbahjat.com)
Date: Tue Jun 25 2002 - 17:43:46 EST


If you simply want fluorescent labeled Staph for uptake studies, you can buy
that directly from Molecular Probes. You'll want to use the BODIPY conjugate
so the signal is not quenched by the acidic pH of the phagolysosome.

You can find the product at:

http://www.probes.com/servlets/product?region=Select+Region&item=2854

I've frequently used this product, as well as the labeled e. coli for
studies of receptor mediated endocytosis.

Good luck

Kb

--
Keith Bahjat, Ph.D.
Applications Engineering Manager
Cytomation, Inc.
Fort Collins, Colorado
keithb@cytomation.com



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