Dear group, In the attached word file (virus free to the best of my knowledge) I am showing two acquisitions of the same file. It was fixed in the 0.75% PFA with 1% HAB serum final in PBS recipe. I don't think its working because the same instrument settings show a huge difference in fluorescent distribution. Does anyone else experience this drag-up with their fixatives? are there any fixatives which don't do this as much?
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