Hello everybody, we start here in our department a clinical trial and have to investigate the activation of monocytes by analysing intracellular cytokine production. Some times ago, I had a similar question about CD-marker phenotyping, but now, in contrast, we want to make functional assays. Therefore, I want to specify my questions: 1) How do you handle the storage / freezing: Is it possible to store whole blood or only the PBMCs? Do you use DMSO or glycerin for storage? Which concentrations of DMSO? 2) Are the results (for example stimulation with LPA: fresh versus stored samples) comparable? Thanks for any suggestions in advance Andreas PD Dr. Andreas Simm Universitaet Halle Wittenberg Klinik fuer Herz- und Thoraxchirurgie Ernst-Grube Str. 40 D-06120 Halle Tel.: +49 (0) 345 557 2647 552 2878 FAX: +49 (0) 345 557 2782 552 2890
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