One approach (both as wash and no wash techniques) that works quite well for intracellular HbF measurements is the method I developed for fetal RBC counting, which is described in these papers: Davis BH, Olsen S, Bigelow NC, Jenn JC: Detection of fetal red cells in fetomaternal hemorrhage using anti-hemoglobin F monoclonal antibody by flow cytometry. Transfusion, 38:749-756, 1998 Chen JC, Bigelow NC, Davis BH: Proposed flow cytometric reference method for erythroid F cells counting. Cytometry, 42:239-46, 2000. Mundee Y, Bigelow NC, Davis BH, Porter JB: Simplified flow cytometric method for fetal hemoglobin containing red blood cells. Cytometry 42:389-393, 2000 Mundee Y, Bigelow NC, Davis BH, Porter JB: Flow cytometric method for simultaneous assay of foetal haemoglobin containing red cells, reticulocytes and foetal haemoglobin containing reticulocytes. Clinical and Laboratory Haematology 23:149-54, 2001 Let me know if it works for your applications. Regards, Bruce H. Davis Maine Medical Center Research Institute Scarborough, Maine >>> BRUEL Sylvie 176694 <BRUEL@dsvidf.cea.fr> 05/15/02 10:31AM >>> Hello, I 'd like to make intracellular labellings in red blood cells so I 'd like to know how can I permeabilise membranes ? Sylvie
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