Hello flow community, who has experience with cell cycle staining of viable cells with Hoechst 33342? The commonly used protocols sound very simple (5-10 µg /ml dye, 30-120 min 37°C). My problem are to high CVs for the particular cell cycle stages, so I can´t sort them probaly. Does anyone know some tricks how to treat the cells (especially Hela cells)? Many thanks in advance Steffen ==================== Dr. Steffen Schmitt FACS Core Facility der Uni Mainz FacsLab Obere Zahlbacher Str. 67 55131 Mainz Phone: + 49 6131 39 30219 Fax: + 49 6131 230506 ==================== www.facslab.toxikologie.uni-mainz.de ====================
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:30 EST