[Solid Tumor dissociation]

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We recently received samples prepared from solid tumors for intracellular
nuclear antigen staining.  Because there were a lot of damages to the
cells and the samples are not suitable for flow analysis.  What can we do
to salvage these samples?  Or, alternatively, what technique is
recommended for dissociation of solid tumors in general for future study.
We have access to Current Protocols in Cytometry.  Can someone comment on
those techniques. Thank you for your help in advance.


Cindy Zhang

Senior Scientist
GlaxoSmithKline
Cellular Pathology
Safety Assessment

Tel: 610 270 4722
Fax: 610 270 7202

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