We have published a convenient 4-color method that does not require the lineage (-) antibodies, used by commercial detection kits. This has allowed us to look at other surface markers in 5th or 6th color. Cytometry 40:50-59, (2000) -----Original Message----- From: Karatza [mailto:mkaratza@otenet.gr] Sent: Monday, April 22, 2002 8:43 AM To: cyto-inbox Subject: dendritic cells Dear flow-ers, Anyone have some information about detecting dendritic cells derived from peripheral blood mononuclear cells by flow ? Any info /direction would be helpful Is autofluorescence a problem?How you get round of it? I have an EPICS ELITE flow cytometer.The monoclonal antibobodies I am going to use are:CD80,CD86 CD83,CD1a,CD14,CD40,HLADR and CD64.
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