Hi flowers,
I posted a query a while ago and received few replies, thinking that some of
you may have been on holiday I'm taking the liberty of re-posting my query.
Advice I have received from Calman (thankyou) points me in the direction of
the PE labelled secondary. What I would really like is someone to let me in
on their incubation times/dilutions of an anti-goat secondary they have used
successfully.
Thanks for your time,
Elaine
>
> Hi All,
>
> We are currently setting up a protocol for indirect flow cytometry on the
> monocytic cell line THP-1.
>
> Our primary antibody is raised in goat and we would really like to find a
> good FITC/PE secondary antibody that has been tried and tested in flow.
>
> Any other tips with regard to dilutions etc would also be greatly
> appreciated.
>
> Many thanks in advance,
>
> Elaine Kennedy
> Pharmacology
> Organon Laboratories Ltd.
> Newhouse
> Scotland
> --------------------------------------------------------------------
> This message, including attached files, may contain confidential
> information and is intended only for the use by the individual
> and/or the entity to which it is addressed. Any unauthorized use,
> dissemination of, or copying of the information contained herein is
> not allowed and may lead to irreparable harm and damage for which
> you may be held liable. If you receive this message in error or if
> it is intended for someone else please notify the sender by
> returning this e-mail immediately and delete the message.
> --------------------------------------------------------------------
--------------------------------------------------------------------
This message, including attached files, may contain confidential
information and is intended only for the use by the individual
and/or the entity to which it is addressed. Any unauthorized use,
dissemination of, or copying of the information contained herein is
not allowed and may lead to irreparable harm and damage for which
you may be held liable. If you receive this message in error or if
it is intended for someone else please notify the sender by
returning this e-mail immediately and delete the message.
--------------------------------------------------------------------
--------------------------------------------------------------------
This message, including attached files, may contain confidential
information and is intended only for the use by the individual
and/or the entity to which it is addressed. Any unauthorized use,
dissemination of, or copying of the information contained herein is
not allowed and may lead to irreparable harm and damage for which
you may be held liable. If you receive this message in error or if
it is intended for someone else please notify the sender by
returning this e-mail immediately and delete the message.
--------------------------------------------------------------------
--------------------------------------------------------------------
This message, including attached files, may contain confidential
information and is intended only for the use by the individual
and/or the entity to which it is addressed. Any unauthorized use,
dissemination of, or copying of the information contained herein is
not allowed and may lead to irreparable harm and damage for which
you may be held liable. If you receive this message in error or if
it is intended for someone else please notify the sender by
returning this e-mail immediately and delete the message.
--------------------------------------------------------------------
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:27 EST