We routinely sort murine bone marrow cells for subsequent culture and/or
injection into immunosupressed mice. Our sort pressure is 40 psi and often
at drop frequencies around 70K. We see good viability and excellent
repopulation following sorting. Also, our stem cell assays are very
similar when we sort at either low pressure (~25) or 40 psi.
|---------+---------------------------->
| | Jeff Barry |
| | <JBarry@picr.man.|
| | ac.uk> |
| | |
| | 09/27/2002 09:33 |
| | AM |
| | |
|---------+---------------------------->
>---------------------------------------------------------------------------------------------------------------|
|
|
| To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
|
| cc:
|
| Subject: Turbo-sorting murine bone marrow
|
>---------------------------------------------------------------------------------------------------------------|
Dear on-line colleagues,
One of our researchers would like to sort murine bone marrow cells at high
speeds but is concerned whether sorting at higher pressure will affect
their
viability. The cells will be put back into lethally irradiated mice for
engraftment and we would like to know the feasibility of this before we
begin the project.
We tend to sort at pressure between 30 - 45 PSI and I recall a previous
posting that said such pressures may cause the suppression of cytokines in
some cell types. Does any one have experience in the high speed sorting of
murine bone marrow and that could shed some light on the subject?
Thanks in anticipation.
Jeff Barry
Flow Cytometry
Paterson Institute for Cancer Research
Manchester
UK
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