Re: Intracellular pH

From: Laura.M.Storck@gsk.com
Date: Mon Sep 30 2002 - 09:03:16 EST

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Hi Rob,

In my hands, I've had good results using the SNARF dye to measure
intracellular pH.  It's manufactured by Molecular Probes and is excited at
488 nm.  I recommend referring to "Current Protocols in Flow Cytometry"
for a good method.

Laura Storck
Scientist
GlaxoSmithKline
King of Prussia, PA






"McCord, Robert" <Rob.McCord@maxygen.com>

26-Sep-2002 18:40




        To:     "Cytometry Mailing List"

        cc:
        Subject:        Intracellular pH


Hello all,

I'm looking for a marker to measure intracellular pH.   Practical Flow
Cytometry (3rd)
mentions both an unnamed UV excited dye used, and BCECF-AM for 488 nm
excitation.
Are these still the most widely used markers?

Does anyone know who may sell them?

Thanks in advance for your help.

Regards,

Rob McCord

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