I would guess that TAT peptides should be the perfect way to achieve that. They are derived from an ancient bacterial transport system through the cell membrane and can be coupled or in this time of molecular techniques simply fused to your molecule of interest. However I think there might already be some patents on that. Regards Gerhard Nebe-von-Caron Research Scientist Applied Science & Technology Group SEAC - Safety and Environmental Assurance Centre Unilever Research, Colworth Laboratory, Sharnbrook, UK - MK44 1LQ Tel: +44 (0)1234 264822, Fax: +44 (0)1234 222552 E- mailto:Gerhard.Nebe-von-Caron@unilever.com -----Original Message----- From: ray hester [SMTP:rhester@JAGUAR1.USOUTHAL.EDU] Sent: Thursday, March 21, 2002 2:20 PM To: Cytometry Mailing List Subject: getting antibodies into viable cells Greetings, A recent discussion on the Confocal Listserv, concerning DAPI staining of viable cells, prompts me to send the following from a colleague: Is there a way to deliver antibodies, or their fragments, into viable cells? We have heard of using liposomes, but wondered if perhaps newer techniques have emerged. Thanks. Ray Hester Univ. of South Alabama Mobile, AL 36688 rhester@jaguar1.usouthal.edu
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