Dear Janet, I don't know if this can help (I'm not in the nematode field) but I do know that there is a dedicated nematode sorter on the market: it was developed by the company of Petra Krauledat and Peter Hansen: I'm sorry I don't recall the company name right now, I think they're located in the Boston area. best regards, Dirk Prof. Dirk Van Bockstaele Laboratory of Hematology Head of Flow Cytometry / Molecular Diagnostics Antwerp University Hospital Wilrijkstraat 10 B-2650 Edegem Belgium phone 32 3 821 3900 fax 32 3 825 1148 > ---------- > Van: janet dow[SMTP:jldow@unity.ncsu.edu] > Verzonden: donderdag 28 februari 2002 16:26 > Aan: Cytometry Mailing List > Onderwerp: Flow on Nematode eggs > > > I have a client who wish to run flow on nematode eggs (Meloidigyne > incognita and Meloidigyne hapla) to stain to look at genome size. We have > tried twice to run them and the first time saw no florescence so we change > protocols to PI but the second time we clogged both my machines with the > samples( I am using a BD FACScan and a FACSCalibur). Can anyone help > us-she has found a paper from 1984 which used flow on a machine made at > Los > Alamos but it doesn't specify what size nozzle they used. She also does > not know how big the eggs are but you can visualize them in the solution > and they settle out of solution very quickly. > > > thank you in advance for all your help-I have always had such great luck > with questions on this group. > > Sincerely > > Janet Dow > > > Janet Dow > Research Technician and Manager > Flow Cytometry Facility > North Carolina State College of Veterinary Medicine > Room C-314 > Raleigh, NC 27606 > (919)513-6364 > >
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