Hi flow-ers, I've got a question referring to quantitative flow cytometry. I wanna measure an antigen in the nucleus and can't decide between FITC and PE for antibody labeling (I wanna use a directly labeled primary antibody). I know there is a lot you can't consider in advance, so as antigen access, impact of fixation and permeabilization methods.....but still - I think PE seems to be more sensitive because of the number of fluorophores each molecule shows, but at the same time the molecule is much bigger, FITC seems to be less sensitive but it is smaller and might have less negative influence on the access to the antigen. What do you think? And, another thing: Does anybody know a good nuclear protein standard I can use? I intended to use histone proteins, but could not find a monoclonal antibody against any of the histones.... Thanks, I really appreciate your help! Lars Brichta University Clinics Bonn, Germany -- +++ GMX - Mail, Messaging & more http://www.gmx.net +++ NEU: Mit GMX ins Internet. Rund um die Uhr für 1 ct/ Min. surfen!
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