Hi, I am new to sorting (but I have ten years of flow experience) and we just purchased a FACSVantage/DiVa. Some of our projects require sorting rare cells at levels of 1 in a million or ten million. Some of the researchers say it could be one in 100 million (which seems quite impossible to me). Does anyone have any pointers or particularly good papers that would help me in this task? How low can you go (in terms of rare events) and still be relatively confident in what you sorted? Currently the researchers I will be doing the sort for has two markers- PI to discriminate live cells and a FITC conjugated marker. I know more markers would be better for discriminating rare events but their doesn't seem to be any for this particular experiment. Any and all help would be greatly appreciated. Todd Todd J. Belanger Lab Manager Cellular Immunology Vaccinex, Inc. 1895 Mt. Hope Ave. Rochester, NY 14620 email: tbelanger@vaccinex.com www.vaccinex.com
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