Dear Emanuel, You are not alone. I have seen this population of cells many times not only in chronic phase of CML but in MDS or CMPD too always as a distinct population (1-8% of the white blood cells). These cells are myeloid cells which have a particular position on the CD45/SSC plot where normally myeloid cells are not presented. The phenotype you described is very consistently myeloid always CD13 and CD33 positive, lacking lymphoid markers and never shows CD34 positivity. Interestingly these cells are in the lymphocyte region on the FSC/SSC plot. After several discussions with my pathologist colleague we concluded that these cells may be some kind of micromyeloblasts. I'm also interested in any knowledge or idea of this great flow community about this myeloid population. Has the presence of these cells any diagnostic or prognostic role in the above mentioned myeloid diseases ? Please let me know if you'll get responses exluding the mine. Best regards, Pal Jakso University of Pecs Faculty of Medicine Dept. of Pathology 7643, Hungary 12. Szigeti str. On Friday, Oct 4, 2002, at 02:52 Europe/Budapest, Emanuel Raniolo wrote: > > Hi Everyone > > Can anyone fill me in on the identity of a cell population that I have > seen > recently in 2 patients with myeloproliferative disorders. > The first patient was a chronic phase CML, the bone marrow of which > displayed a CD45+dim (slightly less than lymphocytes), intermediate > side > scatter (less than monocytes) population on CD45/Log SS analysis. This > population represented approximately 3% of all histogram events. The > phenotype was CD13+ CD33+ CD11b+ CD15- CD14- CD34- CD117-. > The second patient (primary thrombocythemia) bone marrow displayed a > similarly positioned numerically smaller population comprising 1% of > all > histogram events. This patient was not extensively phenotyped, but > again > this population was CD13+ CD34-. > > Has anyone seen these populations previously in their analysis. Do they > represent a mature or immature myeloid population? > > Your responses would be appreciated. Thankyou. > > Emanuel Raniolo > Cell Analysis and Cyopreservation Laboratory > Department of Haematology, IMVS Laboratories > The Queen Elizabeth Hospital Division > Woodville, South Australia. > > >
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