On Mon, 7 Oct 2002 08:46:10 +0200, Pál Jáksó wrote >Pal and Emanuel Have you check if this cells are basophils? In many occasions I found cells like those you described and my conclusion was for basophils because they display this phenotype: intermediate SSC, Cd45 mid, CD33+, Cd34-, CD19-, Cd22+low. Best regards Alessandra Dear Emanuel, > > You are not alone. I have seen this population of cells many times > not only in chronic phase of CML but in MDS or CMPD too always as a > distinct population (1-8% of the white blood cells). These cells are > myeloid cells which have a particular position on the CD45/SSC plot > where normally myeloid cells are not presented. The phenotype you > described is very consistently myeloid always CD13 and CD33 positive, > lacking lymphoid markers and never shows CD34 positivity. Interestingly > these cells are in the lymphocyte region on the FSC/SSC plot. After > several discussions with my pathologist colleague we concluded that > these cells may be some kind of micromyeloblasts. > I'm also interested in any knowledge or idea of this great flow > community about this myeloid population. Has the presence of these > cells any diagnostic or prognostic role in the above mentioned > myeloid diseases ? Please let me know if you'll get responses > exluding the mine. > > Best regards, > Pal Jakso > > University of Pecs > Faculty of Medicine > Dept. of Pathology > 7643, Hungary > 12. Szigeti str. > > On Friday, Oct 4, 2002, at 02:52 Europe/Budapest, Emanuel Raniolo wrote: > > > > > Hi Everyone > > > > Can anyone fill me in on the identity of a cell population that I have > > seen > > recently in 2 patients with myeloproliferative disorders. > > The first patient was a chronic phase CML, the bone marrow of which > > displayed a CD45+dim (slightly less than lymphocytes), intermediate > > side > > scatter (less than monocytes) population on CD45/Log SS analysis. This > > population represented approximately 3% of all histogram events. The > > phenotype was CD13+ CD33+ CD11b+ CD15- CD14- CD34- CD117-. > > The second patient (primary thrombocythemia) bone marrow displayed a > > similarly positioned numerically smaller population comprising 1% of > > all > > histogram events. This patient was not extensively phenotyped, but > > again > > this population was CD13+ CD34-. > > > > Has anyone seen these populations previously in their analysis. Do they > > represent a mature or immature myeloid population? > > > > Your responses would be appreciated. Thankyou. > > > > Emanuel Raniolo > > Cell Analysis and Cyopreservation Laboratory > > Department of Haematology, IMVS Laboratories > > The Queen Elizabeth Hospital Division > > Woodville, South Australia. > > > > > > -- Az. Ospedaliera S.Giovanni Battista di Torino Cap 10126 TORINO C.so Bramante, 88/90
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