What is people's experience in analyzing changes in forms of the so-call 'fluorescent timer' protein? In looking at the fluorescent spectral shift with maturation time, has anyone used a ratiometric technique using red/green? Such a ratio might provide a more sensitive way of showing the relative change in maturation state as well as having the benefit of volume normalization resulting in a more precise assessment of relative cell contents. (Clearly, being able to analyze all time point samples in the same instrument run would be best if the fixation method does not influence the results.) I will post summary to the group unless respondents wish otherwise. Dave ========= David M. Coder, Ph.D. Consultant in Cytometry Seattle, Washington tel./message: 206-499-3446 email: d_coder@msn.com
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