Dako has an anti-FITC antibody. I have used it for immunofluorescence and it works well. I would use a biotin conjugate and then avidin, rather than a direct conjugate. Also, the anti-fitc that I have seen are polyclonal, so plan your controls, because your background will be higher. ron on 5/3/02 12:34 AM, Sathiyaseelan at t.sathi@VERIZON.NET wrote: > > Hi > I have a FITC-conjugated peptide that binds to the surface of a cell line > and gives only a moderate intensity of FL1 signal (in 2nd decade) when > analyzed by flow cytometry. Unfortunately, I do not have a biotin-labelled > peptide in order to use a PE-strepavidin second reagent to amplify the > signal. Could any one tell me if there is a possibility to use an > anti-FITC antibody conjugated to PE as a second reagent to amplify the > fluorescent signal. How much signal amplification I could possibly achieve > using this PE conjugated-anti-FITC antibody method and can I use another > FITC-labeled antibody to label another surface antigen > simultaneously? Does anyone know a source of this antibody? I would > appreciate all useful info and help on this. > > Thanks > SATHI > Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.gov
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