Hi I have a FITC-conjugated peptide that binds to the surface of a cell line and gives only a moderate intensity of FL1 signal (in 2nd decade) when analyzed by flow cytometry. Unfortunately, I do not have a biotin-labelled peptide in order to use a PE-strepavidin second reagent to amplify the signal. Could any one tell me if there is a possibility to use an anti-FITC antibody conjugated to PE as a second reagent to amplify the fluorescent signal. How much signal amplification I could possibly achieve using this PE conjugated-anti-FITC antibody method and can I use another FITC-labeled antibody to label another surface antigen simultaneously? Does anyone know a source of this antibody? I would appreciate all useful info and help on this. Thanks SATHI
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:08 EST