2 questions

From: Beverly E. Barton (bartonbe@UMDNJ.EDU)
Date: Tue May 28 2002 - 10:09:59 EST

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I have 2 questions about fluorochromes for sperm:
1) Has anyone used 7-AAD in place of PI, and if so, what should the
final concentration be in the sample?
2) Has anyone used TMRE in place of JC-1 for sperm, and again what
should the final concentration be?
Thank you very much in advance.
Sincerely,
Beverly

Beverly E. Barton, Ph.D.
Assistant Professor
Department of Surgery
 New Jersey Medical School
 Box 1079
185 S. Orange Ave.
Newark, NJ 07101
phone                973-972-0662
fax                     973-972-6803
e-mail                 bartonbe@umdnj.edu

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